APE 4by4 IF
APE 4by4 IF
APE 4by4 IF is the latest version of combined ELISA and IFA automated processors of DAS. The instrument is able to manage 4 ELISA micro-plates with 10 methods on line or 16 IFA slides with 8 methods on line. All parameter settings for predilution, dispense, incubation, washing, reading and data processing are available. No pre-adjustments or particular calibrations are required. The user's friendly approach for both software and hardware enables the operator to start working with the instrument as soon as it is out of the box.
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Technical features

ELISA

Sample racks     

180 sample tubes of 12mm

Predilution rack 1 tray with 195 tubes
Reagent holders                

modular racks to hold calibrators, controls and reagents for 10 methods on line

Liquid reservoirs

tanks and 4 washing buffer bottles, all equipped with level sensors for a full run capacity

Micro plates

4 plates (96 wells) with incubation capability, from room  temperature to  45°C

Dispense system

X-Y-Z movement with 2 independent needles, each one equipped with its own liquid sensor

Microplate washing

two 8 nozzle on line manifolds able to self-conform to different micro-plates height

Reading system

8 independent photo-metric channels for mono and bichromatic readings

Optical filters 2 filters (450 and 630nm); 2 additional on request (max 4 filters)
Reading inaccuracy ± 1% from 0.000 to 1.500 OD; ± 2% from 1.500 to 3.000 OD
Aspirate System

from 8µl to 1000µl, 1 µl resolution, inaccuracy ± 1% with 100µl
Washer buffers
4 different washing buffers on line
Quality control Integrated

 IFA

Sample racks

180 sample tubes of 12mm 

Predilution racks

2 trays of 108 single cuvettes each. Total number of cuvettes 216 

Reagent holder trays for 8 assays on line, with 2 sample diluents, 15 controls, 7 conjugates, Evans Blue, probe washing solution using the same bottle kits
Liquid reservoirs

external tanks and 1 washing buffer bottle, all equipped with level sensors

Slide tray allotment for 16 slides
Dispense system very accurate and fast X-Y-Z movement with 3 independent needles, 2 to dispense and with its own liquid sensor, the third to aspirate
Slide washing

flow or drop by drop system for single well using 2 needles, one to dispense and the other to aspirate

Aspiration system from 8µl to 1000µl, 1 µl resolution, inaccuracy ± 1% with 100µl
Dispense control and sample  in single or in double
Dilution system

12 dilution ratios programmable by the user, serial dilution into 2 predilution tubes with bypassing option

Settable parameters incubation times for each assay; volume for samples and reagents; slide mask, number of wells and thickness for each slide; shape, size and coordinates for each slide wells;  flow or number of drops for specific washing duration
Printout

worklist, tray configuration, assay setup, undetected liquids

 COMMON FEATURES

Servicing
Easy maintenance
Connection LIS interface capability, work list set up from Host computer. Capability of connecting more instruments toa single PC
Communication port
Bidirectional USB
Power requirements 230/115 VAC, 50-60 Hz, 650W
Measurements 115 x 65 x 65 cm
Weight

103.5Kg

OPTIONAL bar code reader for sample identification